Life Science

DNA Sequencing (Sanger):

Your sequencing lab since 1995. Our principles: Highest possible quality and customer satisfaction.

SEQ Quickview (S001)

DNA sequencing with standard primers or customer primers. You receive the automatically processed sequence data. Depending on the template quality, read lengths of up to 1000 nt per run can be generated. The results are usually available on the same day, at the latest on the following working day after sample receipt.

SEQ Standard (S002)

Manually edited sequence data. In case of laboratory-related problems, the sequencing is repeated without additional costs. The results are usually available on the same day, at the latest on the following working day after sample receipt.

SEQ GC-rich (S004)

Alternative protocol that maximises the success rate of sequencing through regions with moderate GC content. In case of laboratory-related problems, sequencing is repeated at no additional cost. Results are usually available on the same day, at the latest on the following working day after sample receipt.

SEQ Secondary structure (S006)

Newly-optimized protocol for complex sequence regions such as DNA templates with complex secondary structures, long heterohomopolymer regions, palindromic sequences, sequences with tandem repeats or extreme GC regions. In case of laboratory-related problems, sequencing is repeated without additional costs. Results are usually available on the same day, at the latest on the following working day after sample receipt.

SEQ Primer Walking (S008)

Sequencing of one or both DNA strands to determine the unknown sequence of a plasmid DNA or long PCR products, including primer design/synthesis.

SEQ Premixed SEQ (S014)

Fast & economical! Template and primer are already premixed.

SEQ cDNA (S021)

Modified protocol for the sequencing single-stranded cDNA. In case of laboratory-related problems, the sequencing is repeated without additional costs. The results are usually available on the same day, at the latest on the following working day after receipt of the sample.

SEQ RCA Product (S082)

Rolling circle amplification (RCA) is an isothermal method derived from Phi29 bacteriophage replication and has been successfully used by 4base lab in various settings where sample quantity or concentration is limited. RCA is a nucleic acid replication mechanism that can rapidly generate multiple copies of circular pDNA. Therefore, this technique is suitable for the amplification of plasmids at low concentrations and for the direct sequencing bacterial colonies without the need for plasmid preparation. In case of laboratory-related problems, the sequencing is repeated without additional costs. The results are usually available 1-2 days after receipt of the sample.

L-RCA Product (S025)

This service deals with the technical challenges of bidirectional sequencing of amplicon ends or linearised plasmid DNA with homopolymer ends.

DNA sequencing under Pharma/GMP

Information on sample preparation and shipping

Order Form DNA Sequencing

Contamination analysis in disposables for molecular biology

  • DNase-free
  • RNase-free
  • Free of human DNA

Disposables in the life science sector require the highest purity criteria and quality standards. They must be able to be used in molecular biology with the certainty that they neither impair nor falsify laboratory results. All products should therefore be certified as DNase- and RNase-free. In addition, it must be ensured that no contamination with DNA of human origin occurs during production or during the packaging process. At 4base lab, the testing of disposable products for molecular biology is carried out by modern, fast and cost-effective real-time PCR methods.

BAC-DNA-Service

Sale and supply of purified plasmids from human clones:

  • BAC (Bacterial Artificial Chromosome)
  • PAC (P1 Artificial Chromosome)
  • Cosmids
  • Plasmids

BAC-Standard: Obtaining DNA from BAC/PAC clones, without further characterization (incl. UV testing and gel electrophoresis of undigested DNA).

BAC-Verify: Confirmation of identity and genetic stability by restriction digestion, end sequencing or marker PCR.